Informatics Publishing Limited

M. A. Bhat ¹, S. Simon ¹, N. A. Munshi ², Z. A. Bhat ²

Affiliations
1 Department of Plant Protection, Allahabad Agricultural Institute-Deemed University, Allahabad 211007, Uttar Pradesh, IN
2 Sher-e-Kashmir University of Agricultural Sciences and Technology (K), Shalimar Campus, Srinagar 191121, J&K, IN

Abstract

The aim of this study is to determine some effective antagonistic bacterial inoculants for the biological control of Verticillium fungicola causing dry bubble disease of white button mushroom (Agaricus bisporus). Out of the 32 bacterial inoculants isolated from the casing and compost materials and screened against V. fungicola, only 5 isolates were selected for further investigation. The cell free culture filtrates of these bacterial isolates were further evaluated against the pathogen and host fungus at different concentrations. The bacterial culture filtrates inhibited mycelial growth and conidial germination of V. fungicola to varied extents. The isolate PC-I showed maximum inhibition 71.50 per cent of mycelial growth and similar trends of inhibition were recorded in case of conidial germination (84.13 per cent of the pathogen) followed by PS-I, PS-II, Bacillus thuringiensis, BC-IX, BC-V and BC-IV, respectively. Azotobacter sp. proved less effective in inhibiting the mycelial growth of the pathogen. The culture filtrates of PS-I and PS-II stimulated the growth of A. bisporus at all the test concentrations, whereas the remaining culture filtrates inhibited its growth.

Keywords

Bacterial Inoculants, Casing, Compost, In vitro, Verticillium fungicola, White Button Mushroom.

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S. Mohi-Ud-Din ¹, F. A. Zaki ¹, N. A. Munshi ², A. Jan ³, P. Sultan ³

Affiliations
1 Division of Entomology, S. K. University of Agricultural Sciences and Technology, Shalimar Campus, Srinagar, 191121, Jammu and Kashmir, IN
2 Division of Sericulture, SKUAST-K, Shalimar, Srinagar, IN
3 Regional Research Laboratory, Santnagar, Srinagar, IN

Abstract

The pathogenicity of nine fungi was tested in the laboratory against Holotrichia sp. Ail the fungi proved to be pathogenic at a spore concentration 1 × 10⁸ spore/ml to grub with varied mortality. Beaitveria bassiana (Local), B. bassiana (commercial), B. brongniartii and Metarhizium anisopliae were found to be the most effective. B. bassiana @ 1x10⁸ spore/ml concentration inflicted initial mortality on 6^th day (46.6%) and cent per cent mortality was observed on 12^th day. In B. brongniartii initial mortality started on 8^th day (33.33%) and cent per cent mortality occurred on 20^th day, while as in case of M. anisopliae mortality was initiated on 8^th day (30%) and highest mortality was observed on 20^th day (93.91%). In the field, all the three screened bioeontrol agents caused heavy mortality at the highest concentration of 1x10⁸ spore/ml. However, B. bassiana was found most effective as compared to the rest. In case of B. bassiana, the first mortality was observed on 10^th day (36.66%) and cent per cent mortality occurred on 20^th day, while in case of B. brongniartii and M. anisopliae the first mortality was observed on 10^th and 12^th day and complete mortality was observed on 22^nd and 24^th day, respectively.

Keywords

Beaitveria bassiana, Holotrichia sp., Indigenous Fungal Isolates.

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